# flow cytometry # Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) - growth under bubbling stress # PI's: U. Passow, N. D'Souza (USCB), E. Laws (LSU) # version: 2018-10-29 # ========================= Sample_ID Day Treatment Replicate Concentration_cells_mL FSC_Count FSC_Mean FSC_pcnt_CV SSC_Count SSC_Mean SSCpcnt_CV Red_FL_Count Red_FL_Mean Red_FL_pcnt_CV ------------------------- D0-T1 0 No bubbling A 43740 530 1986.82 39.28 530 2691.86 74.70 530 4099.10 89.62 D0-T2 0 No bubbling B 26181 316 2003.20 36.18 316 2865.92 74.38 316 4272.34 43.82 D0-T3 0 No bubbling C 37100 453 1998.68 42.41 453 2744.34 115.01 453 4268.64 79.98 D0-T4 0 Bubbling at 60 ml*min-1 A 41635 512 1952.50 37.32 512 2713.65 93.47 512 4051.36 45.90 D0-T5 0 Bubbling at 60 ml*min-1 B 40219 498 2045.37 49.05 498 2703.81 143.08 498 4294.72 55.83 D0-T6 0 Bubbling at 60 ml*min-1 C 26187 328 1997.46 34.15 328 2757.45 65.68 328 4333.47 45.46 D0-T7 0 Bubbling at 120 ml*min-1 A 31384 395 1975.78 36.19 395 2780.68 107.42 395 4159.01 49.95 D0-T8 0 Bubbling at 120 ml*min-1 B 29413 368 1990.73 37.81 368 2754.93 75.45 368 4227.32 60.59 D1-T1 1 No bubbling A 208673 2641 1816.43 39.52 2641 1837.69 95.86 2641 3015.83 59.45 D1-T2 1 No bubbling B 190344 2389 1914.92 40.67 2389 1943.80 112.88 2389 3265.70 65.00 D1-T3 1 No bubbling C 221736 2802 1816.60 43.13 2802 1878.70 132.17 2802 3069.94 51.94 D1-T4 1 Bubbling at 60 ml*min-1 A 71424 917 1854.71 37.17 917 1916.00 165.93 917 3162.24 58.08 D1-T5 1 Bubbling at 60 ml*min-1 B 144599 1840 1886.30 40.52 1840 1949.76 176.82 1840 3461.87 54.85 D1-T6 1 Bubbling at 60 ml*min-1 C 9916 124 1598.35 46.46 124 1816.10 90.34 124 2640.61 59.33 D1-T7 1 Bubbling at 120 ml*min-1 A 135217 1775 1968.96 41.60 1775 2249.37 147.81 1775 3371.77 51.22 D1-T8 1 Bubbling at 120 ml*min-1 B 178151 2458 2042.23 37.91 2458 3960.25 138.95 2458 3394.99 53.91 D2-T1 2 No bubbling A 569889 7555 2034.13 40.61 7555 2431.68 95.34 7555 2662.79 57.28 D2-T2 2 No bubbling B 618496 8195 2025.61 42.72 8195 2407.17 108.16 8195 2796.98 54.84 D2-T3 2 No bubbling C 599938 7980 1963.20 39.84 7980 2334.48 101.05 7980 2563.42 49.67 D2-T4 2 Bubbling at 60 ml*min-1 A 329301 4439 1697.58 36.04 4439 1545.62 111.81 4439 3136.39 54.08 D2-T5 2 Bubbling at 60 ml*min-1 B 735087 10079 1891.04 37.23 10079 1648.55 145.04 10079 3733.26 50.22 D2-T6 2 Bubbling at 60 ml*min-1 C 28848 361 1396.02 34.16 361 1381.65 66.36 361 2588.61 46.63 D2-T7 2 Bubbling at 120 ml*min-1 A 429640 5735 1879.03 44.54 5735 3089.58 198.56 5735 3441.70 51.50 D2-T8 2 Bubbling at 120 ml*min-1 B 601368 8310 1872.97 44.57 8310 5018.41 158.52 8310 3276.66 50.65 D3-T1 3 No bubbling A 850660 11703 2332.03 45.98 11703 3214.59 86.33 11703 3713.03 52.24 D3-T2 3 No bubbling B 766234 10503 2346.67 45.80 10503 3223.86 80.34 10503 3821.90 51.83 D3-T3 3 No bubbling C 917422 12701 2277.56 47.15 12701 3117.44 87.84 12701 3597.25 51.40 D3-T4 3 Bubbling at 60 ml*min-1 A 826128 11429 2274.32 40.10 11429 1869.38 85.82 11429 5383.42 63.58 D3-T5 3 Bubbling at 60 ml*min-1 B 892761 12397 2769.76 40.20 12397 2558.95 113.11 12397 7811.69 59.21 D3-T6 3 Bubbling at 60 ml*min-1 C 134238 1708 1306.99 43.45 1708 1280.89 262.95 1708 2500.27 52.84 D3-T7 3 Bubbling at 120 ml*min-1 A 933662 13082 2292.86 50.43 13082 4515.37 153.99 13082 3704.64 55.44 D3-T8 3 Bubbling at 120 ml*min-1 B 1077842 15161 2083.64 55.47 15161 5513.65 163.20 15161 3447.89 57.07 D4-T1 4 No bubbling A 778288 10646 2140.95 48.56 10646 3245.04 79.78 10646 4084.66 54.28 D4-T2 4 No bubbling B 662397 9031 2195.87 46.35 9031 3281.79 80.59 9031 4346.80 52.53 D4-T3 4 No bubbling C 791013 10885 2074.70 49.19 10885 3128.75 89.21 10885 3876.73 53.67 D4-T4 4 Bubbling at 60 ml*min-1 A 272850 3732 2477.35 41.69 3732 2628.97 92.78 3732 7428.22 67.70 D4-T5 4 Bubbling at 60 ml*min-1 B 460292 6415 2658.87 50.74 6415 4184.51 101.77 6415 7521.67 82.24 D4-T6 4 Bubbling at 60 ml*min-1 C 326330 4242 1332.90 39.18 4242 1232.28 128.57 4242 2713.91 53.56 D4-T7 4 Bubbling at 120 ml*min-1 A 888022 12427 2225.85 56.25 12427 3770.67 163.97 12427 4193.70 58.11 D4-T8 4 Bubbling at 120 ml*min-1 B 1126789 15799 2020.34 64.32 15799 4566.39 176.13 15799 3803.83 61.02